Abstract
Newcastle disease virus (NDV) has received much attention recently because of its non-specific immune stimulating potential and its various anti-tumor activities. Here we describe that NDV induces synthesis of NO and causes an activation of nuclear factor-kappa B (NF-kappa B) in murine macrophages. These reactions were part of an activation process which included also stimulation of adenosine deaminase and inhibition of 5'-nucleotidase. NDV-mediated NO synthesis and NF-kappa B activation were blocked by an antioxidant (butylated hydroxyanisole), by an inhibitor of protein tyrosine kinase (genistein) and of protein kinase A (H-89), but not by an inhibitor of protein kinase C (staurosporin). These data suggest that signalling requirements of NF-kappa B activation and NO production in NDV-treated macrophages are similar.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Deaminase / metabolism
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Animals
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Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Macrophage Activation
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Macrophages / enzymology
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Macrophages / metabolism*
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Macrophages / virology*
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Mice
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Mice, Inbred DBA
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N-Glycosyl Hydrolases / metabolism
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NF-kappa B / physiology*
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Newcastle Disease / immunology*
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Newcastle Disease / metabolism*
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Newcastle disease virus / immunology*
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Nitric Oxide / metabolism*
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Protein-Tyrosine Kinases / metabolism
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Signal Transduction
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Spleen / chemistry
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Spleen / enzymology
Substances
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NF-kappa B
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Nitric Oxide
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Protein-Tyrosine Kinases
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Cyclic AMP-Dependent Protein Kinases
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N-Glycosyl Hydrolases
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purine nucleosidase
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Adenosine Deaminase