Induction of NO synthesis in macrophages by Newcastle disease virus is associated with activation of nuclear factor-kappa B

Int Immunol. 1996 Apr;8(4):491-8. doi: 10.1093/intimm/8.4.491.

Abstract

Newcastle disease virus (NDV) has received much attention recently because of its non-specific immune stimulating potential and its various anti-tumor activities. Here we describe that NDV induces synthesis of NO and causes an activation of nuclear factor-kappa B (NF-kappa B) in murine macrophages. These reactions were part of an activation process which included also stimulation of adenosine deaminase and inhibition of 5'-nucleotidase. NDV-mediated NO synthesis and NF-kappa B activation were blocked by an antioxidant (butylated hydroxyanisole), by an inhibitor of protein tyrosine kinase (genistein) and of protein kinase A (H-89), but not by an inhibitor of protein kinase C (staurosporin). These data suggest that signalling requirements of NF-kappa B activation and NO production in NDV-treated macrophages are similar.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / metabolism
  • Animals
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Macrophage Activation
  • Macrophages / enzymology
  • Macrophages / metabolism*
  • Macrophages / virology*
  • Mice
  • Mice, Inbred DBA
  • N-Glycosyl Hydrolases / metabolism
  • NF-kappa B / physiology*
  • Newcastle Disease / immunology*
  • Newcastle Disease / metabolism*
  • Newcastle disease virus / immunology*
  • Nitric Oxide / metabolism*
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • Protein-Tyrosine Kinases / metabolism
  • Signal Transduction
  • Spleen / chemistry
  • Spleen / enzymology

Substances

  • NF-kappa B
  • Nitric Oxide
  • Protein-Tyrosine Kinases
  • Cyclic AMP-Dependent Protein Kinases
  • N-Glycosyl Hydrolases
  • purine nucleosidase
  • Adenosine Deaminase