Regulation of inflammation-primed activation of macrophages by two serum factors, vitamin D3-binding protein and albumin

Infect Immun. 1993 Dec;61(12):5388-91. doi: 10.1128/iai.61.12.5388-5391.1993.

Abstract

A very small amount (0.0005 to 0.001%) of an ammonium sulfate [50% saturated (NH4)2SO4]-precipitable protein fraction of alpha 2-globulin efficiently supported inflammation-primed activation of macrophages. This fraction contains vitamin D3-binding protein essential for macrophage activation. Comparative macrophage activation studies with fetal calf serum, alpha 2-globulin fraction, 50% (NH4)2SO4 precipitate, and purified bovine vitamin D3-binding protein revealed that fetal calf serum and alpha 2-globulin fraction appear to contain an inhibitor for macrophage activation while ammonium sulfate precipitate contains no inhibitor. This inhibitor was found to be serum albumin. When bovine serum albumin (25 micrograms/ml) was added to a medium supplemented with 0.0005 to 0.05% (NH4)2SO4 precipitate or 1 to 10 ng of vitamin D3-binding protein per ml, activation of macrophages was inhibited.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Ammonium Sulfate
  • Animals
  • Chemical Precipitation
  • Humans
  • In Vitro Techniques
  • Inflammation / blood*
  • Inflammation / immunology*
  • Macrophage Activation / drug effects*
  • Macrophage Activation / physiology
  • Mice
  • Serum Albumin / immunology
  • Serum Albumin / isolation & purification
  • Serum Albumin / pharmacology*
  • Vitamin D-Binding Protein / immunology
  • Vitamin D-Binding Protein / isolation & purification
  • Vitamin D-Binding Protein / pharmacology*

Substances

  • Serum Albumin
  • Vitamin D-Binding Protein
  • Ammonium Sulfate