19 februari 2018: Bron: J Cancer Res Clin Oncol. 2017; 143(12): 2401–2412. Published online 2017 Sep 23.

Klik op de titel voor het volledige studierapport.

Interessant hoe uitgelegd wordt waarom groene thee extracten (epigallocatechin gallate (EGCG) ) zo goed werken als preventie van kanker en in combinatie met andere medicijnen. Groene thee extracten hebben invloed op de zogeheten kankerstamcellen en bepaalde eiwitexpressie en in het voorkomen en herstellen van RNA mutaties. Bv. in deze studie is de preventieve werking van groene thee extract onderzocht bij darmkankerpatienten met een hoog risico op darmpoliepen en darmkanker:

Zie in gerelateerde artikelen voor meer studies bij kankerpatiënten, maar deze studie is denk ik interessant voor artsen en wetenschappers.

O.a. op deze markers van RNA mutaties en eiwitexpressie heeft een groene thee extract invloed:

Table 1

Human CSCs differentially express stemness markers

Cancers and names of CSCs	Markers of increased expression	Markers of decreased expression	References
Colorectal cancer
HCT116-SDCSCs	mRNAs: Oct4, Nanog,		Toden et al. (2016)
HCT116-SDCSCs	Proteins: CD44, Notch, Bmi-1, CD133, ALDH1
Nasopharyngeal cancer
TW01 sphere	mRNAs: Sox2, Oct4, KLf-4, Twist, Snail, vimentin, N-cadherin	E-cadherin	Lin et al. (2012)
CNE2- & C666-1-SCs	mRNAs: CD44, Bmi-1, N-cadherin, vimentin, Twist	E-cadherin	Li et al. (2015)
CNE2- & C666-1-SCs	Proteins: CD44
Neuroblastoma
BE(2)-C sphere	mRNAs: Nanog, Oct4,	ATP7A, DKK2	Nishimura et al. (2012)
Glioblastoma
U87 GSLCs	mRNAs: ALDH1, CD133,	GFAP	Zhang et al. (2015)
U87 GSLCs	Proteins: CD133, ALDH1,	Cyclin D1

Human cancer stem cells are a target for cancer prevention using (−)-epigallocatechin gallate

Our previous experiments show that the main constituent of green-tea catechins, (−)-epigallocatechin gallate (EGCG), completely prevents tumor promotion on mouse skin initiated with 7,12-dimethylbenz(a)anthracene followed by okadaic acid and that EGCG and green tea extract prevent cancer development in a wide range of target organs in rodents. Therefore, we focused our attention on human cancer stem cells (CSCs) as targets of cancer prevention and treatment with EGCG.

Methods

The numerous reports concerning anticancer activity of EGCG against human CSCs enriched from cancer cell lines were gathered from a search of PubMed, and we hope our review of the literatures will provide a broad selection for the effects of EGCG on various human CSCs.

Results

Based on our theoretical study, we discuss the findings as follows: (1) Compared with the parental cells, human CSCs express increased levels of the stemness markers Nanog, Oct4, Sox2, CD44, CD133, as well as the EMT markers, Twist, Snail, vimentin, and also aldehyde dehydrogenase. They showed decreased levels of E-cadherin and cyclin D1. (2) EGCG inhibits the transcription and translation of genes encoding stemness markers, indicating that EGCG generally inhibits the self-renewal of CSCs. (3) EGCG inhibits the expression of the epithelial-mesenchymal transition phenotypes of human CSCs. (4) The inhibition of EGCG of the stemness of CSCs was weaker compared with parental cells. (5) The weak inhibitory activity of EGCG increased synergistically in combination with anticancer drugs.

Conclusions

Green tea prevents human cancer, and the combination of EGCG and anticancer drugs confers cancer treatment with tissue-agnostic efficacy.

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