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Acknowledgements

The authors would like to thank A. Mixon and S. Farid of the Surgery Branch FACS Core for assistance with data acquisition and cell sorting, and J. Yang and E. Tran for their valuable discussions. This work was supported by the Center for Cancer Research at the National Cancer Institute (NCI) at the US National Institutes of Health (NIH).

Author information

Affiliations

  1. Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    • Nikolaos Zacharakis
    • , Harshini Chinnasamy
    • , Mary Black
    • , Hui Xu
    • , Yong-Chen Lu
    • , Zhili Zheng
    • , Anna Pasetto
    • , Michelle Langhan
    • , Thomas Shelton
    • , Todd Prickett
    • , Jared Gartner
    • , Li Jia
    • , Robert P. Somerville
    • , Paul F. Robbins
    • , Steven A. Rosenberg
    • , Stephanie L. Goff
    •  & Steven A. Feldman

  2. Department of Surgery, Virginia Commonwealth University School of Medicine, Richmond, VA, USA

    • Katarzyna Trebska-McGowan

Contributions

N.Z. designed and performed the experiments, analyzed and interpreted the data, and co-wrote the manuscript; H.C., M.B. and H.X. performed experiments; Y.-C.L., Z.Z. and A.P. designed and performed experiments for single-cell PCR and sequencing for TCR identification and pairing; R.P.S., M.L. and T.S. maintained and developed clinical-grade lymphocytes for patient infusion; P.F.R., T.P., J.G. and L.J. performed and analyzed WES and RNA-seq data for mutation profiling; K.T.-M. (under the direction of S.L.G. and S.A.R.) was responsible for the clinical care of the patient during protocol treatment; S.A.R. conceived the hypothesis, interpreted the data and co-wrote the manuscript; S.L.G. and S.A.F. coordinated the project, analyzed and interpreted data and co-wrote the manuscript.

Competing interests

The authors declare no competing interests.

Corresponding author

Correspondence to Steven A. Rosenberg.